Biomaterials and advanced technologies for hemostatic management of bleeding. What does it take to make the perfect clot? Arterioscler. Thrombin generation and fibrin clot structure. Thrombin generation, fibrin clot formation and hemostasis. Role of proteolytic enzymes in biological regulation (a review). The results provide a mechanistic understanding of how the zeolite surface functionally contributes to thrombin activation, paving the way towards the design of improved agents for bleeding management.
Compared to natural platelet-based physiological processes, prothrombin-to-thrombin conversion on the surface of Ca-zeolite displays a striking thrombin activation pattern, with an exceptionally high plateau thrombin activity and at least 12-fold enhanced endogenous thrombin. Here, we investigate zeolite-initiated thrombin activation at the molecular level, and show that a prothrombinase complex can assemble on the inorganic surface of calcium-ion-exchanged zeolites (Ca-zeolites). However, the mechanism of zeolite-initiated haemostasis has remained unclear. Zeolites comprise a family of crystalline microporous aluminosilicates that show increasing promise for use in massive bleeding control.
Zymogen (prothrombin) activation is central to the process of haemostasis (blood clotting) in the body, preventing serious blood loss and death from haemorrhagic shock.
